Web1. Charge the synthesizer with solvents and detritylation solution needed for the steps shown in Table 3.4.1. These can usually be kept on the synthesizer until they are consumed. 2. Dissolve the protected nucleoside 3 -H-phosphonates (triethylammonium salts) in pyridine and evaporate the solvent (two times) under reduced pressure in a rotary ... WebDichloroacetic acid (DCA) 3% in toluene, BioSyn™ detritylation reagent, for DNA/RNA synthesis, Burdick & Jackson™. Supplier: Honeywell Research Chemicals. Danger. …
Schematic of the detritylation process in the flowcell. Schematic ...
WebIn the legacy process, starting from an empty flowcell, the detritylation solution is filled from the bottom port, resides without mixing (dwell), then drained through the bottom port using gravity. WebNov 2, 2024 · In some cases, using weaker acids during detritylation or reducing acid contact times can be beneficial to maintain low levels of depurination. 1 DCA is a milder acid than TCA, ... Capping is generally achieved using a solution containing acetic anhydride (Cap Mix A) and the catalyst N-methylimidazole (Cap Mix B), ... how healthy is my hair
Detritylation Reagent 674 Honeywell Research Chemicals
WebNational Center for Biotechnology Information WebFigure 2. The detritylation mechanism. The 5'-DMT protecting group is removed by TCA (trichloroacetic acid) in the solvent dichloromethane (too concentrated a solution of TCA or too long of a detritylation … Webdetritylation solution with ESI -MS (Method B). Specifically, a portion of the last batch of detritylation solution was concentrated to dryness and dissolved in methanol (e.g. 2 … how healthy is my period quiz