WebDepositing Lab: Helle Ulrich The AID* Kit is an expanded tool kit for the auxin-inducible degron (AID) system in budding yeast. By expanding the AID degron sequence to include tags for easy detection by antibodies/microscopy, and introducing a set of selection markers, this tool kit provides enhanced flexibility in applying the AID system by facilitating the … Web卡梅德生物(KMD Bioscience)多年来致力于酵母双杂交系统(Yeast two-hybrid system,Y2H)的研究。. 我们的酵母杂交系统是由基于SMART法构建的文库、酵母菌株、严谨的报告基因、高表达载体组成的。. 这项技术可用于:研究蛋白的相互作用;确认蛋白的交互作用;定义 ...
Genome-wide SWAp-Tag yeast libraries for proteome …
WebExpression of the P. falciparum dhfr is controlled by the yeast dhfr 5' and 3' regulatory regions and the heterologous enzyme provided all of the functions of the yeast dhfr gene. These yeast were susceptible to pyrimethamine and cycloguanil at low concentrations that inhibit P. falciparum (IC 50 about 10-8 and 10-7 M, respectively). We constructed plasmids using restriction-free cloning methods36. For a complete list of plasmids, see Supplementary Table 7. The I-SceI restriction site sequence was agttacgctagggataacagggtaatatag. The protein linker sequences (which also served as the generic recombination sites) were as follows: L1, 5′ … See more Total number of genes annotated in SGD currently stands at 6,075, excluding dubious ones. Because of the structure of our tagging cassette, we did not attempt to tag the 62 yeast proteins that include an N′ intron or the 250 … See more The BY4741 laboratory strain38, which is the basis for most systematic yeast libraries, was used as the master strain for the collection. The … See more Donor strains were constructed on the background of an SGA41 compatible query strain and contained a galactose-induced I-SceI … See more To select pristine strains to be included in the final library, we picked four clones for each gene, and performed several quality control steps. Transformations that failed to yield four clones were repeated (462), and those that still … See more phoenix earthquake monitor
Chaperone-like activity of the AAA domain of the yeast Yme1 AAA ...
WebBacterial DHFR enzymes can be effectively targeted while sparing the eukaryotic DHFR due to these sequence changes [47,121]. Recent work examined the evolution of DHFR sequences and identified three crucial areas of human DHFR sequence variation and delineated their impacted on the catalytic mechanism . This study revealed mutations … WebSep 1, 2011 · Because yeast can be cultured under a variety of conditions, including aerobic or fermentative growth, carbon source, temperature and culture density, we investigated … phoenix dry rod oven parts