Webglass-Teflon Potter-Elvehjem homogenizer (Caco-2 cells]. A mixture of proteases inhibitors (1 mM phenylmethylsulfonyl fluoride, 50 wg/ml leupeptin, 50 pg/ml antipain, 0.1 mg/ml aprotinin) was added to all buffer and solutions used for homogenization and membrane purification. Homogenates WebThe Potter-Elvehjem Tissue Homogenizer has a ground glass pestle and tube. They are suitable for processing tougher tissues such as connective tissue. Clearance between the pestle and tube is .004-.006. The Potter …
How do I achieve a proper lysis of mitochondria …
WebJan 16, 2014 · Motor-driven tightly fitting glass/Teflon Potter-Elvehjem homogenizer. Loose-fitting glass/Teflon Potter-Elvehjem homogenizer. Nalgene 30-ml Oak Ridge tubes for the Sigma angled rotor, 6 × 30 ml ... WebNov 24, 2024 · Transfer the cell suspension to a pre-cooled motor-driven tightly fitting glass/Teflon Potter–Elvehjem homogenizer and homogenize with 40 strokes at 1300 rpm (see Note 8). 8. Centrifuge the cell homogenate at 500 × g for 5 min at 4 °C to get a crude nuclear pellet. 9. Prepare aliquots of the supernatant corresponding to 10 mg wet weight. strawberry 19
Isolation of mitochondria-associated membranes and ... - Nature
WebAll glass construction but with a PTFE pestle and an unground tube. Rounded shape on the lower portion of the pestle. Sample is progressively ground smaller in the rounded … WebThe ice-cold homogenate is then transferred to a motor-driven glass/ Teflon potter (Kontes Glass Co.), kept on ice, and 10 full strokes (800 rpm) are applied to the sample. This step breaks up larval tissues releasing intact cells and nuclei. The homogenate is filtered, with the help of the Teflon pestle, through a layer of 64 mm Nitex nylon WebJun 12, 2024 · Cells were then homogenized using a glass/Teflon potter homogenizer. Unbroken cells and nuclei were separated by centrifugation at 600g for 5 min. Further centrifugation of the supernatant at 7000g and 10,000g for 10 min each was performed to isolate the crude mitochondria (pellet), cytosol, microsomes, and plasma membrane … strawberry 1 lb